TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.A.1.19.1
Alkalinizatioin-activated Ca2+-selective channel, sperm-associated cation channel, CatSper, required for male fertility and the hyperactivated motility of spermatozoa (Kirichok et al. 2006). These channels require auxiliary subunits, CatSperβ, γ and δ for activity (Chung et al., 2011).  The primary channel protein is CatSper1 (Liu et al., 2007), and it may be a target for immunocontraception (Li et al. 2009). CatSper channels have been reported to regulate sperm motility (Vicente-Carrillo et al. 2017). Sperm competition is selective for a disulfide-crosslinked macromolecular architecture. CatSper channel opening occurs in response to pH, 2-arachidonoylglycerol, and mechanical force. A flippase function is hypothesized, and a source of the concomitant disulfide isomerase activity is found in CatSper-associated proteins beta, delta and epsilon (Bystroff 2018). More recently, it has been reported that rotational motion and rheotaxis of human sperm do not require functional CatSper channels or transmembrane Ca2+ signaling (Schiffer et al. 2020). Instead, passive biomechanical and hydrodynamic processes may enable sperm rolling and rheotaxis, rather than calcium signaling mediated by CatSper or other mechanisms controlling transmembrane Ca2+ flux. The Ca2+ channel CatSper is not activated by cAMP/PKA signaling but directly affected by chemicals used to probe the action of cAMP and PKA (Wang et al. 2020). The cation channel of sperm (CatSper) is essential for sperm motility and fertility. CatSper comprises the pore-forming proteins CATSPER1-4 and multiple auxiliary subunits, including CATSPERbeta, gamma, delta, epsilon, zeta, and EFCAB9. Lin et al. 2021 reported the cryo-EM structure of the CatSper complex isolated from mouse sperm. CATSPER1-4 conform to the conventional domain-swapped voltage-gated ion channel fold, following a counterclockwise arrangement. The auxiliary subunits CATSPERbeta, gamma, delta and epsilon - each of which contains a single transmembrane segment and a large extracellular domain - constitute a pavilion-like structure that stabilizes the entire complex through interactions with CATSPER4, 1, 3 and 2, respectively. The EM map revealed several previously uncharacterized components, exemplified by the organic anion transporter SLCO6C1. Lin et al. 2021 named this channel-transporter ultracomplex the CatSpermasome. The assembly and organizational details of the CatSpermasome lay the foundation for the development of CatSpermasome-related treatments for male infertility and non-hormonal contraceptives. CatSper is a target for inhibition, for use in male contraception, causing inhibition of sperm motility (Mariani et al. 2023). A CUG-initiated CATSPERθ functions in the CatSper channel assembly and serves as a checkpoint for flagellar trafficking (Huang et al. 2023).

Accession Number:Q9H7T0
Protein Name:Cation channel sperm-associated protein subunit beta aka CatSperβ
Length:1116
Molecular Weight:126924.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate calcium(2+)

Cross database links:

RefSeq: NP_079040.2   
Entrez Gene ID: 79820   
OMIM: 611169  gene
KEGG: hsa:79820   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0030154 P:cell differentiation
GO:0007275 P:multicellular organismal development
GO:0007283 P:spermatogenesis

References (3)

[1] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[2] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[3] “Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.”  Olsen J.V.et.al.   17081983

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence
1:	MESPLIYVSV LLLNIFEFSS GIVYNKDDTE KRFACSNKGF PQENEIIKLY LFLENLKIQC 
61:	FFQTENEIAS KAMLSVFTSG GLAPSLGIMN STYNGIFHFN LTLFSDRILW LVDIPRENIT 
121:	QSTDIAAVEE WLVRITLHHG LNIYATEGTL LDVIREPILQ WTPGDVIPES EISKLYPHVV 
181:	DLKVTKCPCA NDVALLGFIV DTIVDGVYIG ITFGGFWHDY DTTWFNMTQT IYSQLQEEYE 
241:	DLSLVDMVLT NHFLVILTSL GLFVSEDLRY PSRHSLSFSR ADFCGFERVD YVKGKLWYNE 
301:	RCFANREHFE VDYVTVTFER NRTLSESSSC FYSQEPFLEW VPCLPHIFKG IKIFPTVLTF 
361:	LVDQERGTGV YLFYNKVRKT AIASVSTLRN NEPNSQSKFP IFRFPSSFSS PVGMVFHPRS 
421:	HFLYAYGNQI WLSVDGGNTF QLIANFHDDI IKKTFHSFYT SAITFVSQRG KVYSTKAGMG 
481:	RYSAVGSVTE RIFTLYYDHL GFLHKLTLGR FEASGPPTAF GNSRNLFGQP PDMGFETALA 
541:	PQHTSLDEII FFAYVPENEP QETIYSKKFG NIHYGKVIHS GKTGRAYIRK VLQHTTPKGF 
601:	LSSVIAEMKE PFGLEEVNES SCLSSSLLIN KAGNVYKLTL DSQVVQALFE DTDIEKTVVL 
661:	PGYSSFLITS ILDNKNALAI ATMPESAPNN MTFLKSTWFL YNFGQRNGRT WKIYSKPCNY 
721:	WFQHDDSPSL NIVKYIDLGN SYVLKAKVIR NAKGFRMLEI PLLTVFVGNP NLLEVTAEVT 
781:	FDDTDSYVIT ISAASKVLHQ GSTSLAFIMW SASTECFVTT MVPTLKSSCS YLRSMHHIPS 
841:	KFIPFEDWIS GVHKDSQGFN LIKTLPINYR PPSNMGIAIP LTDNFYHADP SKPIPRNMFH 
901:	MSKKTGKFKQ CANVSTREEC NCTKDQKFSH AVAFSDCREK VPRFKFPITQ YPVSLEIINE 
961:	DGRVPLQSPY LVTVTEVNMR HNWKLKHTVP ENIKRMKQLV EPILGAAVYN PSGLNLSIKG 
1021:	SELFHFRVTV ISGVTFCNLI EEFQIYVDEA PLPFPGHTLI AVATAVVLGG LIFIAFMFQL 
1081:	QGIHPWRTFQ RWIRRNQEKF SSISLSELIH RSKSEE